Effects of temperature and various metal ions on the solubility of tobacco RuDP carboxylase

概要

論文の詳細を見る
Tobacco RuDP carboxylase is completely soluble in 0.07 M NaCl or 0.01 M Na_2SO_4, but is almost completely insoluble in salt-free solutions at 40℃; the solubility seeming to depend on ionic strength. Lowering the temperature increased solubility of the protein. The solubility in 0.01-0.04 M NaCl at 0℃ was more than double that at 40℃. RuDP solubilized the protein even in a salt-free medium. The protein became insoluble again on the addition of various divalent cations. Effectiveness of the metal ions was Zn^<++> > Ni^<++> > Co^<++> > Mn^<++> > Mg^<++> > Ca^<++>. Although most of the metal ions inhibited (Mg^<++> activated) enzyme activity, no direct correlation was found between the degree of solubility depression and the degree of enzyme inhibition.
日本植物生理学会の論文