Comparative studies on fraction 1 protein from spinach and tobacco leaves

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Fraction 1 protein of spinach and tobacco leaves was purified by Sephadex G-200 and DEAE-cellulose column chromatography. Immunological comparison of purified preparations of these proteins was carried out with precipitin analysis using an anti-serum of tobacco fraction 1 protein. Two different antigenic activities were found in tobacco fraction 1 protein, of which one was commonly found in the spinach protein and the other was specific to tobacco fraction 1 protein. The immunological results suggest that fraction 1 protein is composed of at least two different structures, one of which is common to both spinach and tobacco proteins and the other of which is specific to each of these proteins. This was confirmed from a chemical experiment. Fraction 1 protein was divided into large and small polypeptide components by sodium dodecyl sulfate treatment and subsequent Sephadex G-100 column chromatography, then the amino acid compositions of each polypeptide of these proteins were compared. The amino acid composition of the small polypeptide of spinach was different from that of tobacco, while amino acid compositions of the large polypeptides of those proteins were similar to each other.
日本植物生理学会の論文