Metabolism of nucleic acids in one-year old apple twig during cold hardening and de-hardening

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Metabolism of nucleic acids in one-year old apple twigs during cold hardening and de-hardening was studied. RNA began to increase one week prior to the rapid increase of cold hardening. Soluble RNA increased 38% in one week, and light and heavy ribosomal RNA's increased 41% in two weeks just prior to and during the stage of rapid cold hardening. DNA followed the same pattern although the concentration was much lower and differences were not as great. A decrease in RNA and an increase in DNA were observed during the slow de-hardening. The most dramatic decrease (40%) occurred in the heavy ribosomal RNA during a three weeks of de-hardening period. MAK is a useful technique for studying nucleic acids which has not previously been adapted for the vegetative tissue of woody species. Nucleic acids from one-year old "Haralson" apple twigs were successfully fractionated into soluble RNA, DNA, and light and heavy ribosomal RNA's. Nine factors involved in the extraction and preparation of nucleic acids were varied and evaluated. The conditions for maximum yield of each class of nucleic acids from apple twigs are also reported.
日本植物生理学会の論文