RIBONUCLEIC ACIDS IN SOLUBLE CYTOPLASM OF GERMINATING BEAN

概要

論文の詳細を見る
From 1.5 day-old bean germ-axes and cotyledons were prepared microsomal (Ms) and 78,400×h supernatant (Sp) RNA's by phenol extraction. Sp-RNA from germ-axes differed considerably from Ms-RNA from either tissue with respect of quantitative nucleotide composition; the former being very similar to yeast s-RNA. Sp-RNA from cotyledons gave an intermediate nucleotide pattern between those of Ms-RNA's and of Sp-RNA of germ-axes. Accepting ability for ^<14>C-leucine in the presence of added ATP, MgCl_2 and "activating enzyme" was the highest in Sp-RNA from germ-axes, followed by Sp-RNA from cotyledons. Ms-RNA's could hardly function as acceptor for activated leucine. The capacity of leucine incorporation of cotyledonous Sp-RNA agreed well with that predicted from its s-RNA level evaluated from the observed nucleotide composition on the assumption that the Sp-RNA consisted of active RNA of yeast s-RNA type and inactive RNA of Ms-RNA type. The results were discussed along the line of a hypothesis on the presence of "transportable RNA" in germinating bean seed tissues.
日本植物生理学会の論文