Comparative Radical Scavenging and Antidiabetic Activities of Methanolic Extract and Fractions from Achillea ligustica ALL.(Pharmacognosy)

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The yield of methanolic extract and total phenol and non polar content of flowered parts from Achillea ligustica ALL. are reported. GC-MS analysis of the non polar fraction showed that the triterpene moretenol was the major constituent (17.228%) followed by stigmast-6-en-3β-ol, veridiflorol and β-amyrin (7.524%, 5.078% 4.470%, respectively). The antioxidant activities of the methanolic extract and its fractions from A. ligustica were carried out using two different in vitro assays, 2, 2-diphenyl-1-picrylhydrazyl (DPPH) test and lipid peroxidation of liposomes assay. Methanolic extract showed higher radical scavenging activity on DPPH (IC_<50> of 50μg/ml). This activity is probably due to the phenolic fraction which shown an IC_<50> value of 22μg/ml. A different result was obtained from the methanolic extract on the lipid peroxidation of liposomes (IC_<50> of 416μg/ml). The α-amylase inhibition assay was applied to evaluate antidiabetic activity. The methanolic extract showed weak activity (28.18% at 1mg/ml) while the n-hexane fraction showed 74.96% inhibition at 250μg/ml.
2005-09-01