A Fluorometric Rate Assay of Peroxidase Using the Homovanillic Acid-o-Dianisidine-Hydrogen Peroxide System
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概要
- 論文の詳細を見る
The oxidation of homovanillic acid by peroxidase in the presence of hydrogen peroxide is stimulated by various compounds. Among the compounds tested, o-dianisidine was found to stimulate the oxidation of homovanillic acid strongly. A rapid and highly sensitive method for the fluorometric rate assay of peroxidase using o-dianisidine as an oxidative stimulator in the homovanillic acid-hydrogen peroxide system was established. By this method, it is possible to determine peroxidase activity in the range of 0.5-18 mU/ml.
- 社団法人日本薬学会の論文
- 1983-10-25
著者
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石原 文子
Division Of Chemistry Of Hygiene Meiji College Of Pharmacy
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石原 文子
1st Department of Biochemistry, Meiji College of Pharmacy
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秋浜 澄行
1st Department of Biochemistry, Meiji College of Pharmacy
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岩井 広行
1st Department Of Biochemistry Meiji College Of Pharmacy
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岩井 広行
麻布大学
関連論文
- Evidence for the Presence of Two Kininogens in Porcine Plasma and the Isolation of High-Molecular-Weight Kininogen
- A Fluorometric Rate Assay of Peroxidase Using the Homovanillic Acid-o-Dianisidine-Hydrogen Peroxide System
- Electric Microassays of Glucose, Uric Acid and Cholesterol Using Peroxidase Adsorbed on a Carbon Electrode
- オルトジアニシジン-ペルオキシダーゼ系によるホモバニリン酸の測定
- Peroxidase吸着炭素電極による過酸化水素の測定