Evidence for the Presence of Two Kininogens in Porcine Plasma and the Isolation of High-Molecular-Weight Kininogen

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When the pseudoglobulin fraction of porcine plasma was gel-filtered through a column of Sephadex G-150,two fractions, from which kinin was released on incubation with trypsin [EC3.4.21.4], were observed. This indicates the presence of two kininogens with different molecular weights in the plasma. The larger-molecular-weight kininogen fraction had the ability to correct the abnormal clotting time of human high-molecular-weight (HMW) kininogen-deficient plasma, Fitzgerald plasma. The smaller-molecular-weight kininogen fraction did not show this ability. Thus, the fraction showing the ability to correct the abnormal clotting time of Fitzgerald plasma was considered to contain a kininogen corresponding to that purified as an HMW kininogen from bovine, human, horse and rat plasma. The other fraction may contain porcine low-molecular-weight (LMW) kininogen.The porcine HMW kininogen was isolated by a modification of the purification method for horse HMW kininogen. The ability of the isolated HMW kininogen obtained to correct the abnormal clotting time of Fitzgerald plasma was in proportion to the amount of the preparation used. When this HMW kininogen preparation was incubated with purified porcine plasma kallikrein [EC 3.4.21.8], the release of bradykinin was observed.
社団法人日本薬学会の論文
1986-11-25