Isoquinoline系化合物の制癌性に関する研究(第5報) : 1-(2-Methylbutyl)-3-methyl-6,7-methylenedioxyisoquinoline・HClのSarcina luteaおよびHeLa細胞の核酸, 蛋白合成におよぼす影響

概要

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Effect of an anti-tumor compound, 1-(2-methylbutyl)-3-methyl-6,7-methylenedioxyisoquinoline・HCl (B-15) on nucleic acids and protein synthesis of Sarcina lutea and synchronized HeLa cells was investigated ; the following results were obtained. A selective inhibitory activity against the incorporation of ^3H-thymidine into DNA of Sarcina lutea was observed with B-15 at concentrations which were not, or only slightly, inhibitory in the bacterial viability test. On the other hand, the rapidly labeled DNA in the nucleus of Sarcina lutea was not degraded. In synchronized HeLa cells, the incorporation of ^3H-thymidine into DNA was inhibited immediately after addition of B-15 and the extent of inhibition was greater than in the case of the incorporation of ^3H-uridine into RNA or ^<14>C-leucine into proteins. Moreover, if added at the stage of DNA synthesis, B-15 inhibited both the incorporation of ^3H-thymidine into DNA and division of the cells. However, the inhibition of DNA synthesis was rapidly reversed when B-15 was removed from the culture medium. From these results, it seems that the primary action of B-15 on the intact cells is a selective inhibition of DNA synthesis.
公益社団法人日本薬学会の論文
1969-07-25