溶着薄層クロマトグラフィー(第11報)溶着薄層形成におよぼす固着剤ガラスのpHの影響

概要

論文の詳細を見る
Unlike soda-lime glass powder, borosilicate glass powder fails to give homogeneous thin-layers when used as a binder for the preparation of silica gel sintered plates. Examination of pH of water suspensions of these two kinds of glass powder revealed that the borosilicate powder has lower pH than the soda-lime glass powder, suggesting that pH of the glass might affect the formation of thin-layers. Addition of sodium methoxide or ammonium hydroxide to the suspension of silica gel-borosilicate glass powder in various solvents was found to increase sedimentation volume of the glass powder. Among several kinds of solvent tested, acetone was the most effective in increasing the sedimentation volume. Thus, the silica gel-borosilicate glass powder suspension in acetone with sodium methoxide or ammonium hydroxide added gave, after usual processing, homogeneous thin-layer plates which exhibited normal chromatographic separation of various organic compounds such as alkaloids, estrogens, and other steroidal hormones. Addition of basic flocculants and use of acetone were also effective for the preparation of alumina-borosilicate and kieselguhr-borosilicate sintered plates.
公益社団法人日本薬学会の論文
1974-09-25