Radioimmunoassay法によるProstaglandinの分離定量に関する検討(第2報)二重展開法による各Prostaglandinの相互分離とProstaglandin E_2の測定

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An examination was made for individual separation of prostaglandins (PGs) from living tissues or body fluid. Total PGs, extracted by the previously reported procedure^<1)> were separated into PG E_1,E_2,F_<1a>, and F_<2a> by a stepwise development method applied to thin-layer chromatography on 5% (w/v) silver nitrate-sprayed silica gel HR plate after first development for separating to PGs E and PGs F. Rabbit PG E_2 antiserum, which has a high specificity to PG E_2,was also obtained. With the use of this antiserum, radioimmunoassay capable of measuring low levels of PG E_2 was developed. It was concluded from the results of PG levels measured by the individual separation method and the group separation method reported previously that each prostaglandin should be measured by the individual separation method.
公益社団法人日本薬学会の論文
1973-04-25