ラット肝臓ミトコンドリヤのプロトヘムフェロリアーゼの精製
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From the rat liver mitochondria, protoheme ferro-lyase [E. C. 4.99.11], an enzyme localized on its inner membrane, was purified about 44-fold over the specific activity of the homogenate. The purification was carried out by (NH_4)_2SO_4 fractionation, gel filtration on Sepharose-4B, column chromatographies on DEAE-cellulose and hydroxylapatite in the presence of detergents. From the results of DISC and SDS-polyacrylamide gel electrophoresis, this enzyme was estimated to be an oligomer enzyme with an approximate molecular weight of 380000 consisting of NA-H-1,an extrinsic protein on the inner membrane, and D-1 and D-2,intrinsic proteins linked by a hydrophobic bond to the inner membrane, and the approximate molecular weights of these subunit proteins were 120000,dimer of 74000 and 110000,respectively.
- 社団法人日本薬学会の論文
- 1981-03-25
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