ろ紙採血法を用いた血中イセパマイシン濃度測定法の小動物への応用

概要

論文の詳細を見る
In general, collection of serial blood samples from small experimental animals is difficult in terms of sampling site and operation technique. To overcome these problems, a simple reproducible method has been improved by the use of filter papers. Whole blood obtained by venipuncture from the ear vein of guinea-pig was spotted onto a filter paper. Isepamicin in the dried blood spot was extracted with 0.5 M Na_2HPO_4 buffer by incubation and determined by fluorescence polarization immunoassay. Linearity was established over the range of 5-150 μg/ml by using only 100 μl of whole blood. Consequently its accuracy and precision were good, with mean coefficient of variation of less than 5%. The method described here correlates well with a conventional sampling method and could be used for the pre-clinical study of isepamicin blood levels of individual guinea-pigs. This method is suitable for the simulataneous measurement of aminoglycoside antibiotics or physiological parameters after the administration of aminoglycoside antibiotics for the pharmacokinetics study.
社団法人日本薬学会の論文
1989-12-25