並列比濁時間分析法によるエンドトキシンの自動測定
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概要
- 論文の詳細を見る
An attempt has been made to develop an automated instrument that measures the turbidity change in gelation reaction of a solution containing endotoxin and Limulus amoebocyte lysate, and quantifies endotoxin by determining gelation time of the sample. This instrument monitors the ratio R (t) of the sequential to the initial transmittance of up to 64 samples simultaneously and independently at 10 s increments. As the samples are stationarily incubated at controlled temperature of 37±0.5℃, the objective judgement of gelation is provided without any disturbance from sample vibration. Defining gelation time as the time required to obtain 5% decrease of R (t), the well correlated calibration curve was obtained for endotoxin concentration from 1 pg/ml to 100 ng/ml. The coefficients of variation of the calculated endotoxin concentration were 5.64 to 14.1%. The judgement of gelation by this method agreed well with that by the conventional gel-clot method when the proper measuring time (about a half of that by the conventional method) was selected.
- 公益社団法人日本薬学会の論文
- 1985-03-25
著者
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大石 晴樹
Osaka Research Laboratory, Wako Pure Chemical Ind., Ltd.,
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畑山 泰道
Osaka Research Division, Wako Pure Chemical Ind., Ltd.,
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佐方 由嗣
Osaka Research Division, Wako Pure Chemical Ind., Ltd.,
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柳沢 和也
和光純薬工業(株)大阪研究所
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大石 晴樹
和光純薬工業(株) ME 開発部 ME 研究室
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畑山 泰道
和光純薬工業(株)大阪研究所
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白石 浩己
和光純薬工業(株)大阪研究所
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佐方 由嗣
和光純薬工業(株)大阪研究所
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佐方 由嗣
Osaka Research Division Wako Pure Chemical Ind. Ltd.
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大石 晴樹
Osaka Research Laboratory Wako Pure Chemical Ind. Ltd.
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畑山 泰道
Osaka Research Division Wako Pure Chemical Ind. Ltd.
関連論文
- Discrimination between Endotoxin and (1→3)-β-D-Glucan Using Turbidimetric Kinetic Assay with Limulus Amebocyte Lysate
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