スペクトロメトリーを利用した微量生体関連物質の分離分析法の開発と応用
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概要
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Recent studies on the development and application of analytical methods in HPLC with spectrometric detection for bio-related compounds have been reviewed. First, new fluorescent derivatization reagents with benzofurazan skelton have been developed. In the course of the study, the relationship between fluorescent characteristics and substituent groups of benzofurazan compounds was investigated. The relationship enabled to design new fluorogenic reagents easily Secondly, the simultaneous determination method for the sequence and D/L configuration of peptides with fluorescent Edman reagent was developed. In this study, the racemization mechanism of amino acids in the cleavage/cyclization step was investigated and boron trifluoride was employed as cleavage/cyclization reagent in order to supress the racemization. Finally, sensitive and selective determination system for catecholamine with peroxyoxalate chemiluminescence detection after derivatization with ethylenediamine was developed. Utilizing this system, plasma catecholamine level of spontaneously hypertensive rats and Wistar-Kyoto rats after administration of calcium antagonists were measured, and the attenuation of the baroceptor reflex was investigated.
- 公益社団法人日本薬学会の論文
- 1998-11-01
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関連論文
- 「薬学教育と薬剤疫学タスクフォース」報告書 : 薬学教育の中の薬剤疫学の位置づけに関するアンケート調査
- D-アミノ酸研究の最近の進歩
- 微量物質(内分泌撹乱物質以外の微量生体物質)
- スペクトロメトリーを利用した微量生体関連物質の分離分析法の開発と応用
- 国立大学における「長期課題研究及びアドバンスト教育プログラム」の現状