アルコール連続投与による肝アルデヒド脱水素酵素活性の変動とマウス系統差について

概要

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Properties of hepatic aldehyde dehydrogenase (ALDH), a key enzyme for controlling the detoxication of alcohol, were compared in two strains of mice with different sensitivities to alcohol. The ALDH activities were also compared in four experimental animals including mice, rats, guinea pigs and rabbits, and humans under the same conditions. The activities were different among the animal species and strains. The enzyme activities were mainly localized in the cytosolic fraction in all animals tested. However, the subcellular distribution pattern of ALDH was different among the animals. Molecular properties of ALDH in hepatic cytosolic fraction of the animals were analysed by isoelectric focusing. The results indicated that each animal had a characteristic pattern. Under successive treatments of ethanol to two strains of mice, genetically different in the sensitivity to alcohol, ALDH activities and isozyme patterns were compared. In the alcohol sensitive strain, DBA/2J, ALDH activities in subcellular fractions did not change by giving 5% ethanol to this strain for 20 d. On the other hand, in the ethanol-resistent strain, C57BL/6J, given 10% ethanol as drinking water for 20 d, ALDH activities were elevated in microsomal and cytosolic fractions and the isozyme pattern of the cytosolic fraction underwent marked changes related to the treatment.
公益社団法人日本薬学会の論文
1989-02-28