Determination of Food Preservatives by High-Performance Liquid Chromatography

概要

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A new and simple method is presented for simultaneously determining the preservatives in foodstuffs, such as benzoic acid, sorbic acid, and ethyl, isopropyl, propyl, isobutyl, and butyl esters of p-hydroxybenzoic acid. This method is characterized by the achievement of ethanol extraction and clean-up of the extract at a single step. The target compounds were determined by high-performance liquid chromatography. The sample was homogenized with ethanol, and the extract was injected onto a reverse-phase colume (ODS packing) with a mixture of methanol-0.4 M acetate buffer, pH 4.6 (45 : 55) as the mobile phase. The effluent was monitored with a UV detector at a wavelength of 240 nm. The preservatives in complicated matrices such as fermented foods have been determined with recoveries of more than 95%.
公益社団法人日本薬学会の論文
1985-02-28