高速液体クロマトグラフィーによる大気浮遊粉じん中のベンゾ(α)ピレンの簡易分析法

概要

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A simplified analysis of benzo (α) pyrene (BαP) in airborne particulates was investigated by a high performance liquid chromatography (HPLC). Airborne particulates were collected on a glass fiber filter (20×25 cm) by a Hi-volume air sampler and a part of the filter (diameter ; 14 mm) was placed on a column filled with 2g of basic alumina. BαP in the particulate was extracted by an eluting solvent of ethanol and benzene. The eluate was completely evaporated under reduced pressure and then the residue was redissolved in acetonitrile. BαP was analyzed by a reversed phase HPLC equipped with a spectrofluorometer. Recovery of BαP and its coefficient of variation by this method were 98.7% and 1.84%, respectively. when this method was applied to the determination of BαP in airborne particulates, the analytical data were in good agreement with those by conventional method ; ultrasonic extraction-HPLC method. Polynuclear aromatic hydrocarbons are well separated by a reversed phase column. Extraction and clean-up are performed simultaneously to simplify the procedure and save time for the analysis. The advantage of HPLC using a spectrofluorometer is its sensitivity and selectivity.
1983-02-28