ラット肝顆粒画分中のカドミウム

概要

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Male Wistar rats were injected with 1.5 mg Cd/kg/day subcutaneously for six days. Four days after the last injection, the liver tissues were removed and homogenized. The homogenate was centrifuged at 27000×g for 60 min and the resultant precipitate was homogenized and further centrifuged twice. Surfactants such as Triton X-100,sodium deoxycholate and sodium dodecyl sulfate solubilized a Cd-containing component from the precipitate. The solubilized Cd-containing component was gelfiltrated on Sephadex G-75. Cadmium in the subcellular particle was mainly eluted as metallothionein.
公益社団法人日本薬学会の論文
1980-04-30