カドミウムによる腎障害発現と細胞内挙動

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The present investigation was undertaken to study a possible relationship between a change in the binding form of Cd in kidney cells and renal damage in Cd poisoning. 1) Twenty male rats were divided into four injection dose groups of 0,0.3,1.0,and 1.5 mg Cd/kg body weight. Subcutaneous injections were given for six days per week. After 4 weeks, renal tubular dysfunction in the 1.0 and 1.5 mg dose groups was indicated by glycosuria and decreased renal leucine aminopeptidase activities. 2) Kidney tissues were homogenized separately in 4 volumes of cold 0.25 M sucrose and the homogenates were centrifuged at 27000×g for 60 min. The 27000×g supernatant was fractionated on Sephadex G-75. The major portion of Cd in the supernatant in the 0.3mg dose group was detected as bound to a low molecular weight protein, metallothionein (F-2) and, therefore, Cd was assumed not to be toxic to the kidney. The concentration of Cd increased in all fractions in the 1.5mg dose group and the rate of increase in the higher molucular weight fraction (F-1) was greater than that in metallothionein (F-2). 3) Increased dosage of Cd resulted in an increase in the accumulation of Cd in the 27000×g sediment. It is assumed that this sediment contains nuclei, mitochondria, lysosomes, and heavy microsomes. 4) These findings suggest that the elevation of the concentration of Cd both in the higher molecular weight fraction (F-1) among 27000×g supernatant fraction and in the 27000×g sediment fraction may be related to the development of renal dysfunction.
公益社団法人日本薬学会の論文
1978-04-30

カドミウムによる腎障害発現と細胞内挙動

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