Determination of Glucuronic and Ascorbic Acid in Oral and Pharmaceutical Preparations by Isotacophoresis

概要

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A simple and rapid analytical method for the determination of glucuronic and ascorbic acid in oral and pharmaceutical preparations was developed. This method not only showed good results for organic acids including citric, tartaric, malic, fumaric, latic, succinic, acetic and aspartic acid in oral and pharmaceutical preparations but also in health drinks. The detection limits of this method are about 0.04 to 0.1 mg within the coefficient of variation of about 6%. The entire analytical procedure can be accomplished within 40 min. without pretreatment. Using this method, a leading electrolyte solution was prepared with 7 parts of 0.1% polyvinyl alcohol in 0.01 N HCl, buffered at pH 3.0 with β-alanine and 3 parts of methanol. A solution of 0.01 N n-caproic acid was used as the terminal electrolyte. There was a two stage migration procedure : the first stage in a 10 mm i.d.×10 cm tube with 250 μA current for 10 min, and the second stage in a 5 mm i.d. 15 cm tube with 100 μA current for 10 min. The oven temperature was kept at 25℃. The nitrogen carrier gas was introduced at 0.25 kg/cm^2 pressure.
公益社団法人日本薬学会の論文
1996-02-29