Homozygous Deletion of Mouse Homolog of p16/CDKN2 Gene on Chromosome 4 in Mouse Liver Epithelial Cells in Culture

スポンサーリンク

概要

• 論文の詳細を見る

• The culture of hepatocytes isolated from C3H mouse liver results in the spontaneous development of colonies of liver epithelial cells that possess some features of the hepatocytes. These liver epithelial cells frequently have a loss of chromosome 4,and become neoplasms which are hepatocellular carcinomas by transfection with the activated c-Ha-ras gene. The suppression of malignant phenotypes by mouse chromosome 4 has already been shown by fusion between normal and malignant mouse cells. We established a total of six liver epithelial cell lines from C3H mice in order to investigate the presence of a tumor suppressor gene(s) on chromosome 4 in mouse hepatocarcinogenesis, and performed an allelotype analysis in seven microsatellites on chromosome 4 by the comparative multiplex PCR method. The result of analysis revealed that three of the six liver epithelial cells had allelic imbalances in four microsatellite loci, especially, two liver epithelial cell lines showed homozygous deletion in the D4MIT77 locus. Then, we investigated the status of the mouse homolog of p16/CDKN2 gene (mouse p16) on chromosome 4 by the comparative multiplex PCR method, and detected the homozygous deletion in two liver epithelial cell lines. Our result thus supports the theory that alterations of tumor suppressor gene(s) located on chromosome 4 may play a role in mouse hepatocarcinogenesis. Mouse p16,which is an inhibitor of cyclin dependent kinase 4,may suppress the cancer development in mouse hepatocarcinogenesis, or suppress liver cell immortalization.

• 公益社団法人日本薬学会の論文
• 1996-03-15

著者

• Kawauchi Saju

  Department Of Hygienic Chemistry Hoshi University

• MIYASAKA Kazuyoshi

  Department of Hygienic Chemistry, Hoshi University

• Miyasaka K

  Department Of Pathology Cancer Institute:department Of Hygienic Chemistry Hoshi University

• Miyasaka Kazuyoshi

  Department Of Hygienic Chemistry Hoshi University:department Of Pathology Cancer Institute

関連論文

• Allelotype Analysis in Mouse Hepatocellular Carcinomas; Frequent Homozygous Deletion of Mouse Homolog of p16/CDKN2 Gene on Chromosome 4 in Culture
• Susceptibility of Day-12.5 and Day-13.5 Fetal Mouse Palates Cultured in Vitro to 5-Fluorouracil and Hydroxyurea
• Radiographic Analysis of Lumbar Motion in Relation to Lumbosacral Stability : Investigation of Moderate and Maximum Motion
• Homozygous Deletion of Mouse Homolog of p16/CDKN2 Gene on Chromosome 4 in Mouse Liver Epithelial Cells in Culture
• EFFECTS OF CAFFEINE AND RELATED METHYLXANTHINES ON FETAL MOUSE PALATES CULTURED IN VITRO
• Scoliosis Associated With Airflow Obstruction Due to Endothoracic Vertebral Hump

スポンサーリンク