EFFECTS OF CAFFEINE AND RELATED METHYLXANTHINES ON FETAL MOUSE PALATES CULTURED IN VITRO

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概要

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• The maxillary regions of day-12.5 ICR mouse fetuses were cultured in a chemically-defined serumless medium and the effects of methylxanthine derivatives on cultured palates were studied. Explanted palates were treated for 72 hr in vitro with 0.5-2 mM caffeine (CA), 1-2 mM theophylline (TP), or 1-2 mM theobromine (TB). Although the three compounds tested did not prevent the contact of opposing palatal shelves, palatal fusion was inhibited by CA and TP at a concentration of 2 mM, and the inhibitory effect of CA was more evident than that of TP. On the other hand, TB did not exert an inhibitory effect on palatogenesis at 2 mM. Since the in vitro toxicity of the methylxanthine compounds appeared to correlate well with their in vivo teratogenic potential, the organ culture method of fetal rodent palates should be a useful tool for screening teratogenic agents, especially those causing cleft palate.

• 日本トキシコロジー学会の論文
• 1994-11-30

著者

• Kawauchi S

  Hoshi Univ. Tokyo Jpn

• Kawauchi Saju

  Department Of Hygienic Chemistry Hoshi University

• KOSAZUMA Tsuneo

  Institute of Applied Medicine, Hokkaido Radio-Isotope Center, Ltd.

• Kosazuma T

  Department Of Anatomy And Developmental Biology And Congenital Anomaly Research Center Kyoto Univers

関連論文

• Organ culture of the fetal mouse palate for screening the developmental toxicity of chemicals : A validation study
• Susceptibility of Day-12.5 and Day-13.5 Fetal Mouse Palates Cultured in Vitro to 5-Fluorouracil and Hydroxyurea
• Homozygous Deletion of Mouse Homolog of p16/CDKN2 Gene on Chromosome 4 in Mouse Liver Epithelial Cells in Culture
• EFFECTS OF CAFFEINE AND RELATED METHYLXANTHINES ON FETAL MOUSE PALATES CULTURED IN VITRO

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