Effect of Benzylidene Derivative, Novel Antirheumatic Agent, on the Production of Tissue Inhibitor of Metalloproteinases

スポンサーリンク

概要

• 論文の詳細を見る

• We investigated the effects of PAL (protocatechualdehyde), a metabolite of ACP (3,4-diacetoxy benzylidene diacetate) which is a candidate as a novel antirheumatic agent, on the production of a tissue inhibitor of metalloproteinases (TIMP) using a primary chondrocyte culture from bovine nasal septum cartilage.The addition of human recombinant interleukin-1α (hrIL-1α) induced proteoglycan (PG) depletion from a chondrocyte matrix. PAL significantly reduced the induction of PG depletion. HrIL-1α increased the casein-degrading activity, matrix metalloproteinase (MMP) activity, and the level of TIMP secretion in the culture media. PAL significantly reduced the casein-degrading activity and further increased TIMP secretion under hrIL-1α stimulation. Phorbol myristate acetate (PMA) also increased the level of TIMP secretion, and staurosporine, a specific inhibitor of protein kinase C (PKC), reduced the TIMP secretion to the control level under hrIL-1α or PMA stimulation. Furthermore, PAL showed a significant increase following treatment with a low concentration of PMA which alone did not increase TIMP production. These findings suggest that the activation of the PKC pathway plays an important role in TIMP production and that PAL increases the level of TIMP production through an additive or synergistic effect with the activation of the PKC pathway.In conclusion, these findings demonstrate that the inhibition of the MMP activity and the increase of TIMP production by PAL contribute to the inhibition of PG depletion in a primary chondrocyte culture from bovine nasal septum cartilage.

• 公益社団法人日本薬学会の論文
• 1994-01-15

著者

• 森 陽

  Department of Biochemistry, Tokyo College of Pharmacy

• 森 陽

  Department Of Biochemistry Tokyo College Of Pharmacy

• 渡辺 孝寿

  Biomedical Research Laboratories, Kureha Chemical Industry Co., Ltd.,

• 林 治久

  Biomedical Research Laboratories, Kureha Chemical Industry Co., Ltd.,

• 林 治久

  Biomedical Research Laboratories Kureha Chemical Industry Co. Ltd.

• 渡辺 孝寿

  呉羽化学工業(株)生物医学研究所

• 渡辺 孝寿

  Biomedical Research Laboratories Kureha Chemical Industry Co. Ltd.

関連論文

• Stimulation of the Biosynthesis of Interleukin 8 by Interleukin 1 and Tumor Necrosis Factor α in Cultured Human Chorionic Cells
• Anti-ulcer Effects of Lactic Acid Bacteria and Their Cell Wall Polysaccharides
• Effect of Bleomycin on the Synthesis of Hexosamine-containing Substances in Cultured Fibroblasts
• 26,27-DIETHYL-1α,25-DIHYDROXYVITAMIN D_3 AND 24,24-DIFLUORO-24-HOMO-1α,25-DIHYDROXYVITAMIN D_3 : HIGHLY POTENT INDUCER FOR DIFFERENTIATION OF HUMAN LEUKEMIA CELLS HL-60(Communications to the Editor)
• Dipeptidase Activity in Rat Carrageenin Granuloma
• Purification and Characterization of an Acid Proteinase from Human Uterine Cervix
• Isolation and Partial Characterization of Hyaluronidase and Exo-polysaccharidases in Rat Carrageenin Granuloma
• Matrix metalloproteinases 産生阻害薬(CPA-926)のOAモデルにおける軟骨変性抑制作用
• Effect of Benzylidene Derivative, Novel Antirheumatic Agent, on the Production of Tissue Inhibitor of Metalloproteinases
• Effect of High-Molecular-Weight Hyaluronic Acid on the Viscosity of Rabbit Pericardial Fluid Measured with a Cone and Plate Viscometer

スポンサーリンク