Detection of Tetracycline Resistance Protein Encoded by Bacillus subtilis Plasmid pNS1
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概要
- 論文の詳細を見る
Tetracycline resistance protein (TET) of Bacillus subtilis plasmid pNS1 was detected by immunoblot analysis using a specific antibody to TET-chloramphenicol acetyltransferase (CAT) fusion protein. In two-dimensional electrophoresis, one major spot which seemed to be the pNS1-encoded TET (pNS1-TET), was detected by immunostaining. Its molecular weight and isoelectric point were approximately 52kDa and 6.2,respectively. Judging from the nucleotide sequence, the pNS1-TET is a very hydrophobic, 50kDa protein. Therefore, the 52kDa protein is thought to be an intact form of the pNS1-TET produced by B. subtilis cells.
- 公益社団法人日本薬学会の論文
- 1993-01-15
著者
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青木 隆
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkai
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渡部 博之
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido
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青木 隆
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Health Sciences University Of Hokkaido
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金田 光弘
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Higashi-Nippon-Gakuen University
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金田 光弘
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Higashi-nippon-gakuen University
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