Cloning and Characterization of the Promoter Region of Monkey Thymidylate Synthase Gene

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概要

• 論文の詳細を見る

• Thymidylate synthase (TS) catalyzes the conversion of deoxyuridylate to thymidylate. Promoter regions of the TS gene from three vertebrates, human, mouse and rat, have been analyzed so far. A unique inverted repeat was present in the promoter region of the human, but not in the mouse and rat, TS genes, and the feature of the promoter region is considerably different between the human TS gene and the two rodent TS genes. To examine whether the characteristics of the human TS gene promoter is intrinsic to human or primate, we cloned a DNA fragment containing the promoter region of the monkey TS gene and determined the nucleotide sequence in this region. The nucleotide sequence in the promoter region of the moneky TS gene was revealed to have 88% homology with that in the corresponding region of the human TS gene. The unique repeted structure mentioned above was also found in the monkey TS gene. Furthermore, the Sp1-binding motif and the CACCC box were found to be conserved in the monkey TS gene. The result of chloramphenicol acetyltransferase assay suggested that the Sp1-binding motif and the CACCC box are essential for the promoter activity of the monkey TS gene, as in the case of the human TS gene.

• 公益社団法人日本薬学会の論文
• 1998-10-15

著者

• TAKEISHI Keiichi

  Laboratory of Genetic Engineering, School of Food and Nutritional Sciences, University of Shizuoka

• Takeishi Keiichi

  Laboratory Of Genetic Engineering Graduate School Of Nutritional And Environmental Sciences And Scho

• Horie Nobuyuki

  Laboratory Of Genetic Engineering School Of Food And Nutritional Sciences University Of Shizuoka

• WAKABAYASHI Maki

  Laboratory of Genetic Engineering, School of Food and Nutritional Sciences, University of Shizuoka

• KATAI Hideyuki

  Laboratory of Genetic Engineering, School of Food and Nutritional Sciences, University of Shizuoka

• Wakabayashi M

  Laboratory Of Genetic Engineering School Of Food And Nutritional Sciences University Of Shizuoka

• Katai Hideyuki

  Laboratory Of Genetic Engineering School Of Food And Nutritional Sciences University Of Shizuoka

関連論文

• Transcriptional Regulation by C/EBPα and -β in the Expression of the Gene for the MRP14 Myeloid Calcium Binding Protein
• Synergistic Effect of Green Tea Catechins on Cell Growth and Apoptosis Induction in Gastric Carcinoma Cells(Analytical Biochemistry)
• Cloning and Characterization of the Promoter Region of Monkey Thymidylate Synthase Gene
• Implication of CDK Inhibitors p21 and p27 in the Differentiation of HL-60 Cells(Biochemistry/Molecular Biology)
• Characterization of Nuclear Factors that Bind to the Human Thymidylate Synthase Gene in HL-60 Cells Differentiated by All-trans Retinoic Acid Treatment
• Functional Analysis and DNA Polymorphism of the Tandemly Repeated Sequences in the 5'-terminal Regulatory Region of the Human Gene for Thymidylate Synthase

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