Implication of CDK Inhibitors p21 and p27 in the Differentiation of HL-60 Cells(Biochemistry/Molecular Biology)

スポンサーリンク

概要

• 論文の詳細を見る

• All-trans retinoic acid (ATRA) differentiates HL-60 cells into granulocyte-like cells and cellular proliferation is repressed markedly along with the morphological and physiological changes specific for cellular differentiation. To elucidate the implication of cyclin-dependent kinase (CDK) inhibitors during differentiation, we examined the expression of CDK inhibitors during the differentiation of HL-60 cells. The expression of p21 and p27 among the CDK inhibitors we examined increased during the differentiation induced with ATRA. Then, we established stable transformants of HL-60 cells expressing antisense RNA for p21 and p27 and examined the ability of these cells to differentiate into granulocyte-like cells. The extents of fully differentiated HL-60 cells transfected with genes for antisense RNA of p21 and p27 were only 53% and 60%, respectively, whereas 90% of the parental HL-60 cells differentiated by the ATRA treatment. These results suggest that increased expression of CDK inhibitors, p21 and p27, is necessary for the differentiation of HL-60 cells induced with ATRA.

• 公益社団法人日本薬学会の論文
• 2004-07-01

著者

• Ishikawa Aki

  Laboratory Of Genetic Engineering Graduate School Ofnutritional And Environmental Sciences Universit

• Asada Hidetsugu

  Laboratory Of Genetic Engineering Graduate School Ofnutritional And Environmental Sciences Universit

• Takeishi Keiichi

  Laboratory Of Genetic Engineering Graduate School Of Nutritional And Environmental Sciences And Scho

• Mori Tsuyoshi

  Laboratory Of Genetic Engineering Graduate School Ofnutritional And Environmental Sciences Universit

• Horie N

  Laboratory Of Genetic Engineering Graduate School Ofnutritional And Environmental Sciences Universit

• Horie Nobuyuki

  Laboratory Of Genetic Engineering School Of Food And Nutritional Sciences University Of Shizuoka

• Takeishi K

  Laboratory Of Genetic Engineering Graduate School Ofnutritional And Environmental Sciences Universit

• JOHNSTON Patrick

  Department of Oncology, The Queens University of Belfast

• Johnston Patrick

  Department Of Oncology The Queens University Of Belfast

関連論文

• Transcriptional Regulation by C/EBPα and -β in the Expression of the Gene for the MRP14 Myeloid Calcium Binding Protein
• Two pedigrees of hereditary prostate cancer
• Synergistic Effect of Green Tea Catechins on Cell Growth and Apoptosis Induction in Gastric Carcinoma Cells(Analytical Biochemistry)
• Cloning and Characterization of the Promoter Region of Monkey Thymidylate Synthase Gene
• Implication of CDK Inhibitors p21 and p27 in the Differentiation of HL-60 Cells(Biochemistry/Molecular Biology)
• Characterization of Nuclear Factors that Bind to the Human Thymidylate Synthase Gene in HL-60 Cells Differentiated by All-trans Retinoic Acid Treatment
• Functional Analysis and DNA Polymorphism of the Tandemly Repeated Sequences in the 5'-terminal Regulatory Region of the Human Gene for Thymidylate Synthase

スポンサーリンク