Formation of Active Oxygen Species from Diethyistilbestrol, a Synthetic Estrogen, and Its Metabolite in the Presence of RAW 264.7 Cells(Toxicology)

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Previously, we reported that (±)-IA but not DES produces O^-_2 spontaneously in PBS. We are interested in the possibility that these compounds might produce active oxygen species under mild cell culture conditions. On incubation of RAW 264.7 cells with (±)-IA the signal of 5,5'-dimethyl-1-pyrroline-N-oxide (DMPO)-OH adducts increased but no more than the additive effect. However, stimulation of RAW cells with LPS and INF-γ enhanced the formation of DMPO-OH adducts slightly more than the additive effect, especially when the concentration of (±)-IA increased. In the case of DES, the spectra of DMPO-OH adducts did not increase concentration-depen-dently in the absence of RAW 264.7 cells, however in their presence, they increased concentration-dependently, especially when these cells were stimulated with LPS and IFN-γ. The results were interpreted to mean that DES would have a higher oxidation potential than (±)-IA not be oxidized to semiquinoes spontaneously, and therefore not produce DMPO-OH adducts in the absence of RAW cells. In their presence, DES might be easily oxidized to semiquinones by the reaction with O^-_2 produced from RAW 264.7 cells.
公益社団法人日本薬学会の論文
2002-10-01