Effects of Various Antihypertensive Drugs on the Function of Osteoblast

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概要

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• Several studies have suggested that high blood pressure is associated with the risk of bone loss. Since various antihypertensive drugs are in wide use for the treatment of hypertension, it is important to investigate the influences of these drugs on bone metabolism. Osteoblasts play a pivotal role in the regulation of bone formation. During differentiation, they sequentially express type I collagen, alkaline phosphatase(ALP), other bone matrix proteins, and finally undergo mineral deposition. In this study, we examined the effects of various antihypertensive drugs on the function of osteoblast using clonal MC3T3-E1 cells. Drugs examined include dihydropyridinetype calcium channel blokers(benidipine, amlodipine, and nifedipine), angiotensin-converting enzyme(ACE)inhibitors(captopril, lisinopril, and enalapril), and angiotensin II receptor type1(AT1)antagonists(TCV-116 and KW-3433). None of the ACE inhibitors or AT1 antagonists affected ALP activity or cellular DNA content signifilantly. In contrast, binidipine, amlodipine and nifedipine increased ALP activity when used in amounts 1_<pM> 100_<nM> and 100_<nM> respectively. Benidipine blocked calcium influx through the L-type voltage dependent calcium channel of MC3T3-E1 more porently than amlodipine or nifedipine. These calcium channel blockers did not change collagen accumulation. Benidipine significantly increased in vitro mineralization at a concentration of 1_<nM> and higher, wyile amlodipine did so at 1μ_M and nifedipine did not. Comparison of the effective concentration of each calcium channel blocker in our study with the reported maximum serum concertration of each drug suggests that benidipine, but not amlodipine or nifedipine, promotes mineral deposition in human.

• 公益社団法人日本薬学会の論文
• 2001-06-01

著者

• Sugimoto S

  Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.

• Sugimoto Seiji

  Tokyo Research Laboratories Kyowa Hakko Co. Ltd.

• Nishiya Yoichi

  Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.

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• Effects of Eel Neuropeptide Y on Ion Transport Across the Seawater Eel Intestine
• Large-scale Purification and Refolding of Recombinant Eel Growth Hormone(Biological Chemistry)
• Effects of Various Antihypertensive Drugs on the Function of Osteoblast

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