Using Polymerized C9 to Produce a Monoclonal Antibody against a Neoantigen of the Human Terminal Complement Complex
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概要
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The terminal complement complex (TCC), consisting of C56,C6,C7,C8,and C9,contains neoantigens that are absent from the individual native components. Neoantigens are present both in the membrane-bound (MAC) and the fluid-phase (SC5b-9) complex. The present study describes production of monoclonal antibodies (MoAbs) against neoantigens of both forms of the TCC using human C9 polymerized in Zn^<2+> as an immunogen. One of ten MoAbs obtained, MoAb 1B4,reacted with the tubular C9 polymer, but not with either the native or sodium dodecyl sulfate-denatured monomeric C9,as shown by enzyme-linked immunosorbent assay and Western blotting. Moreover, MoAb 1B4 cross-reacted both with MAC and SC5b-9. This suggests MoAb 1B4 recognized a neoantigen in the moiety of C9 polymer in the TCC. MoAb 1B4 will be of value for definitive identification of MAC and for quantitation of SC5b-9 in plasma and urine.
著者
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Kusunoki Yukihiro
Department Of Oral And Maxillofacial Sciences Gifu University School Of Medicine
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NAGASAWA Shigeharu
Department of Pharmaceutical Sciences, Hokkaido University
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Nagasawa S
Department Of Pharmaceutical Sciences Hokkaido University
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TAKEKOSHI Yasuo
Department of Pediatrics, School of Medicine, Hokkaido University
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Nagasawa Shigeharu
Department Of Hygienic Chemistry Faculty Of Pharmaceutical Sciences Hokkaido University
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Takekoshi Yasuo
Department Of Pediatrics School Of Medicine Hokkaido University
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Kusunoki Yukihiro
Department Of Pediatrics School Of Medicine Hokkaido University
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