A HYPOALBUMINEMIC SUBSTANCE FROM EHRLICH ASCITES CARCINOMA CELLS

概要

論文の詳細を見る
A hypoalbuminemic substance was purified from mouse Ehrlich ascites carcinoma cells by ammonium sulfate fractionation, adsorption on DEAE-cellulose, gel filtration on Sephadex G-200,treatment with butanol, ultracentrifugation, and gel filtration on Sephadex G-75. Electrophoresis of the substance on polyacrylamide gel containing sodium dodecylsulfate gave a single band which corresponded to a molecular weight of 53000. It showed hypoalbuminemic, hypoproteinemic, reticuloendothelial system-stimulating, and leukocyte-lowering effects when given to mice by intraperitoneal injection. Of these effects, only the leukocyte-lowering effect disappeared when it was administered to adrenalectomized mice. The vascular permeability-increasing activity concentrated into the fraction unadsorbed on DEAE-cellulose and distinctly separated from the hypoalbuminemic activity. The purified substance was composed of 28.9% of DNA, 24.9% of hexose, 24.6% of RNA, 14.6% of amino acid residues, and 1% of hexosamine. The hypoalbuminemic activity was decreased markedly by the partial digestion with Pronase, trypsin, or DNase, whereas the treatment with RNase or the butanol extraction exerted no influence on the activity.
公益社団法人日本薬学会の論文