Interactions of Cyclodextrins with Dipalmitoyl, Distearoyl, and Dimyristoyl Phosphatidyl Choline Liposomes. A Study by Leakage of Carboxyfluorescein in Inner Aqueous Phase of Unilamellar Liposomes
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概要
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The interaction of cyclodextrins (CDs) with L-α-dipalmitoyl phopsatidyl choline (DPPC), L-α-distearoyl phosphatidyl choline (DSPC), and L-α-dimyristoyl phosphatidyl choline (DMPC) unilamellar liposomes was investigated by the leakage of carboxylfluorescein (CF) entrapped in the inner aqueous phase of liposomes, at 25℃(DPPC and DSPC liposomes) and at 5℃ (DMPC liposomes). The efficiency of CDs for CF leakage was remarkable in the order of heptakis (2,6-di-O-methyl)-β-CD (DOM-β-CD)>α-CD>heptakis (2,3,6-tri-O-methy)-β-CD (TOM-β-CD) from DPPC liposomes, in the order of DOM-β-CD> TOM-β-CO> : α-CD from DSPC liposomes and in the order of α-CD>DOM-β-CD<TOM-β-CD from DMPC liposomes. The other CDs used in the present studies, β-CD, 2-hydroxylpropyl β-CD, and γ-CD scarcely induced the CF leakage from above the three liposomes.From the profiles of % CF leakage, together with measurements of differential scanning calorimetry, it was found that hydrophobic DOM-β-CD penetrates the matrix of the liposomes to interact with them as well as TOM-β-CD, and that less hydrophobic α-CD exists at the surface of the membrane to interact with the liposomes. Further, it was found that the interaction of CDs with liposomes changes depending not only on the length of fatty acid chain of phospholipid (condensation force and hydrophobicity) but also the hydrophobicity and the cavity size of CD.
- 社団法人日本薬学会の論文
- 2000-01-01
著者
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Yoshida Junko
Kobe Pharmaceutical University
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NISHIJO Juziro
Kobe Pharmaceutical University
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SHIOTA Sachiko
Kobe Pharmaceutical University
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MAZIMA Kaori
Kobe Pharmaceutical University
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INOUE Yukiko
Kobe Pharmaceutical University
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MIZUNO Hitomi
Kobe Pharmaceutical University
関連論文
- Interactions of Cyclodextrins with Dipalmitoyl, Distearoyl, and Dimyristoyl Phosphatidyl Choline Liposomes. A Study by Leakage of Carboxyfluorescein in Inner Aqueous Phase of Unilamellar Liposomes
- The Interaction of 1-Naphthalenesulfonate with β-Cyclodextrin. Studies by Calorimetry and Proton Nuclear Magnetic Resonance Spectroscopy
- Interaction of Heptakis (2, 3, 6-Tri-0-methyl) -β-cyclodextrin with Cholesterol in Aqueous Solution
- Interactions of Cyclodextrins with DPPC Liposomes. Differential Scanning Calorimetry Studies
- Interactions of Cholesterol with Cyclodextrins in Aqueous Solution
- ADSORPTION OF VARIOUS TYPE CATIONS ON NEGATIVELY CHARGED LIPOSOME
- Interaction of 2-Naphthalenesulfonate with β-Cycldextrin : Studies with Calorimetry and Proton Nuclear Magnetic Resonance Spectroscopy
- The Solid Complex of Aminomalonic Acid with β-Alanine and Its Thermal Decomposition