On the Heterogeneity of Eukaryotic Ribosomal Subunits in Vitro

スポンサーリンク

概要

• 論文の詳細を見る

• The circumstances in which monomers and dimers of eukaryotic small and large ribosomal subunits occur were examined by measuring the sedimentation profiles of each subunit with glutaraldehyde fixation. When small ribosomal subunits were prepared from rat liver ribosomes in the medium containing 50mM Tris-HCl (pH 7.6), 880mM KCl, 12.5mM MgCl_2,10mM mercaptoethanol and 0.1mM puromycin, about 41.4% of the preparations sedimented as a monomer and the other as a dimer in T_<10>K_<20>M_5 (10mM Tris-HCl, pH 7.6 ; 80mM KCl ; 5mM MgCl_2) buffer. There were no difference in the monomer content of small ribosomal subunits from free and membrane bound ribosomes from rat liver. Moreover, small ribosomal subunits from the muscle of normal and diabetic rats contained just the same proportion of the monomer and dimer particles when assayed under the same ionic conditions. The proportion of the monomer and the dimer of the small ribosomal subunits changed with change in the concentration of potassium and magnesium at which ribosomal subunits were prepared ; the content of the monomer decreased with lowering the ratio of magnesium concentration and was only 10.1% when the preparation was conducted in the medium containing 880 mM KCl and 3mM MgCl_2. The addition of poly U to the small subunit preparations caused the conversion of the dimer into the monomer. Large ribosomal subunits dimerized gradually at low temperature even in high potassium concentration but the dimers of the large subunits dissociated easily into their monomers by incubation at 30°.

• 公益社団法人日本薬学会の論文
• 1976-05-25

著者

• 中谷 一泰

  School of Pharmaceutical Sciences, Showa University

• 中谷 一泰

  School Of Pharmaceutical Sciences Showa University

関連論文

• Induction of Differentiation of Human Leukemia Cells by Various Combinations of Cytokines and Low-Molecular-Weight Inducers
• Effects of Vitamin D_3 and Related Compounds on Angiotensin Converting Enzyme Activity of Endothelia Cells and on Release of Plasminogen Activator from Them
• Characterization of Cell-Surface Material Released from AH-66 Hepatoma Ascites Cells into an Isotonic Solution
• Isolation of Serine, Glutamic Acid, and Glycine-Rich Nonhistone Proteins from Bovine Cerebral Cortex
• On the Heterogeneity of Eukaryotic Ribosomal Subunits in Vitro

スポンサーリンク