Sensitivity to Antitumor Drugs and Vinblastine Binding to Membrane in Rat Ascites Hepatoma AH66 Cells

概要

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Rat ascites hepatoma AH66 cells have lower sensitivity to Vinca alkaloids and anthracycline antibiotics than AH66F cells, a subline of AH66 cells. AH66 cells expressed P-glycoprotein, while the protein was not detectable in AH66F cells. There are two affinity sites for [^3H]vinblastine binding in the AH66 cell membrane, while AH66F cells have only one affinity site. The high affinity [^3H]vinblastine binding in AH66 cells was inhibited by Adriamycin, verapamil, nicardipine, and reserpine. The high affinity site of the binding may be the multidrug transporter, P-glycoprotein. [^3H]Vinblastine binding was not influenced by adenosine 3', 5'-monophosphate (AMP), adenosine triphosphate (ATP), or guanosine triphosphate (GTP). The multidrug resistance in AH66 cells may depend on P-glycoprotein which is not modulated by nucleotide.
社団法人日本薬学会の論文
1992-08-25