High Performance Liquid Chromatographic Determination of Bound Sulfide and Sulfite and Thiosulfate at Their Low Levels in Human Serum by Pre-column Fluorescence Derivatization with Monobromobimane
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概要
- 論文の詳細を見る
A sensitive and specific high performance liquid chromatographic method for the determination of sulfide, sulfite, and thiosulfate was established. Inorganic sulfur anions were converted into fluorescent derivatives with monobromobimane. The derivatives were separated on a coupled column chromatography with a reversed-phase octadecyl silica column connected with a weakly basic anion exchanger column by isocratic elution with acetic acid solution (pH 3)-acetonitrile (13 : 3,v/v) containing 25 mM NaClO_4. The method was applied to the determination of bound sulfide and sulfite and thiosulfate in normal human serum. Thiosulfate could be determined directly by use of an ultrafiltered sample. For the determination of bound sulfide and sulfite, the pretreatment step with continuous flow gas dialysis was effective for the sample after releasing sulfide and sulfite by reduction with dithiothreitol. The limits of quantification by the present method were 0.05 μM for thiosulfate, 0.5 μM for bound sulfide, and 0.2 μM for bound sulfite.
- 社団法人日本薬学会の論文
- 1992-11-25
著者
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川鍋 康治
Departments Of Analytical Chemistry Meiji College Of Pharmacy
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田辺 信三
明治薬科大学分析化学
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兎川 忠靖
明治薬科大学 分析化学教室
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兎川 忠靖
Departments of Analytical Chemistry, Meiji College of Pharmacy
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小川 真弘
Departments of Analytical Chemistry, Meiji College of Pharmacy
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縄田 正志
Departments of Analytical Chemistry, Meiji College of Pharmacy
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小笠原 裕樹
Hygienic Chemistry, Meiji College of Pharmacy
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田辺 信三
Hygienic Chemistry, Meiji College of Pharmacy
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小笠原 裕樹
明治薬大
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Ogasawara Y
Department Of Hygienic Chemistry Meiji College Of Pharmacy
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田辺 信三
明治薬大
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Tanabe Shinzou
Department Of Hygienic Chemistry Meiji College Of Pharmacy
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縄田 正志
Departments Of Analytical Chemistry Meiji College Of Pharmacy
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小川 真弘
Departments Of Analytical Chemistry Meiji College Of Pharmacy
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小笠原 裕樹
明治薬科大学衛生化学
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- High Performance Liquid Chromatographic Determination of Bound Sulfide and Sulfite and Thiosulfate at Their Low Levels in Human Serum by Pre-column Fluorescence Derivatization with Monobromobimane
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