Self-Association of Telomeric Short Oligodeoxyribonucleotides Containing a dG Cluster

概要

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Oligonucleotides containing a dG cluster, d(T_mG_nT_m), are models of single-stranded parts of telomeric deoxyribonucleic acid and substitutes for poly(dG). Electrophoretic and spectroscopic analyses of the oligomers indicate that the oligomers can form two alternative structures, single- and quadruple-stranded helices, in solution at room temperature. The transformation of the single-stranded form into the quadruple-stranded form or vice versa is undetectable in 0.1 M NaCl at 4.3×10^<-5> M strand concentration at room temperature. However, at a 50-fold higher strand concentration, the single-stranded oligomer is gradually converted into the quadruplex. An increase in ionic strength stabilizes the single-stranded structure, so it seems to inhibit the formation of the quadruplex. The quadruplex, [d(TTGGGGTT)]_4,is resistant to denaturation in 7 M urea, in which the Watson-Crick type d(TTGGGGTT)・d(AACCCCAA) duplex dissociates. An increase in the number of T residues facilitates the dissociation of the quadruplex by heating. Thus the number of T residues surrounding the dG cluster might control the rigidity of the quadruplex structure.
公益社団法人日本薬学会の論文
1991-09-25