Formycin A Resistant Mutants Due to Defect in Adenosine Transport System in Vibrio parahaemolyticus

概要

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An antibiotic formycin A inhibited growth of Vibrio parahaemolyticus under certain conditions, which suggested that formycin A was taken up by cells under these conditions. We found that formycin A was transported via the adenosine transport system which we previously reported as a Na^+-coupled contransport system. We isolated many formycin A resistant mutants, and about half of them grew very poorly on adenosine as a sole source of carbon. Judging from their reversion frequencies, these mutants seemed to have single mutations. Respiration driven uptake of ^<14>C-adenosine was not observed in such mutants; also, Na^+ uptake induced by the addition of adenosine or formycin A to a cell suspension was completely abolished in them. Thus we conclude that these mutants possess a defect in the Na^+/adenosine cotransport system, and have become formycin A resistant.
社団法人日本薬学会の論文
1991-05-25