Age- and Exercise-Related Changes in the Activities of Thiol Proteinases in Rat Skelatal Muscle

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The activities of thiol proteinases and their endogenous inhibitors in musculus rectus femoris were studied in control and exercised rats of various ages. Cathepsins B and H, both lysosomal thiol proteinases, showed defferent age-ralated changes in activity. Two forms of calcium-activated neutral protease (CANP), ^μCANP and mCANP, differ from the lysosomal thiol proteinases with ragard to age-related changes in activity. The activities of endogenous inhibitors of CANP, cathepsins B and H are apparently not affected by age. Among CANP, cathepsins B and H, only the activity of CANP (the sum of ^μCANP and mCANP activities) in the 16-week-old rats was reduced after training. Exercise had no effect on the activities of endogenous inhibitors in the 16-week-and 98-week-old animals. The relative percentage of the activity of ^μCANP to the activities of ^μCANP plus mCANP did not change regardless of differences of age or exercise. These findings suggest that factors other than endogenous inhibitors may be inbolved in the age- and exercise-related changes in the activities of these thiol proteinases, and also that there is a close relationship between ^μCANP and mCANP in skeletal muscle.
公益社団法人日本薬学会の論文
1988-10-25