Effect of Rhatannin on Glutamine Metabolism in Rat Liver

概要

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The present investigation was undertaken to evaluate the effect of rhatannin (condensed tannin purified form Rhei rhizoma) on transamination from glutamine by measuring the individual amino acids formed from α-keto analogues of amino acids with glutamine or glutamane or glutamate in the supernate of rat liver homogenate. Increased glycine formation was observed 2 to 4h after the intraperitoneal administration of rhatannin (12.5mg/kg body weight). Glutamate (derived from glutamine via glutaminase) may not participate in the rhatannin-induced elevation of glyoxylate amination by glutamine. This effect by rhatannin was completely abolished by cycloheximide treatment 30 min prior to rhatannin treatment, while actinomycin D had no effect. With 20 mM glutamine as the amino-donor, the order of effectiveness of 20 mM keto analogues of amino acids was glyoxylate>pyruvate>hydroxypyruvate>phenylpyruvate>α-ketoisocaproate, and little activity was observed with α-ketoisovalerate. Phenylalanine and leucine production rates were enhanced with either glutamine or glutamate in rhatannin-treated rats.The results of the present investigation suggest that the hepatic glutamine transaminase pathway might be enhanced in rhatannin-treated rats. This enhancement might be associated with a protein synthesis process.
社団法人日本薬学会の論文
1986-07-25