Characteristics of Augmentation of Cell-Mediated Cytotoxic Activities by Recombinant Human Interferon-Gamma (Met-Glu Form) : A Comparative Study with Natural Human Interferon-Alpha and -Beta

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概要

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• The effects of recombinant human interferon (IFN)-gamma (Met-Gln form), which has the same amino acid sequence as natural human IFN-gamma except for the N-terminal methionine, on various cell-mediated cytotoxicities were studied in vitro comparatively with those of natural human IFN-alpha and -beta. The augmentations of natural killer (NK) and antibody-dependent cell-mediated cytotoxic (ADCC) activities against tumor cells by IFN-gamma (Met-Gln form) were equivalent or somewhat inferior to those by the natural IFNs. However, the augmentation of ADCC activity against chicken red blood cells (CRBCs) by IFN-gamma (Met-Gln form) was superior to those by natural IFNs. This prominent effect of IFN-gamma (Met-Gln form) was considered to be mediated by monocytes contained in the mononuclear cell preparation used as effector cells. As expected, IFN-gamma (Met-Gln form) actually augmented antibody-dependent monocyte-mediated cytotoxicity against CRBCs more effectively than the natural IFNs. IFN-gamma (Met-Gln form) also enhanced macrophage-mediated tumor cytotoxicity, like other preparation of recombinant IFN-gamma. The NK-augmentation by IFN-gamma (Me-Gln form) was further increased by combination with indomethacin, and the effect was completely reversed by further addition of prostaglandin E_2 (PGE_2). However, these agents had no effect on the NK-augmentation by natural IFN-beta. These results suggest that IFN-gamma (Met-Gln form) activates macrophages (rather than NK and killer (K) cells) to kill tumor cells and release PGs more effectively than IFN-alpha and -beta.

• 公益社団法人日本薬学会の論文
• 1986-11-25

著者

• 松本 修

  Basic Research Laboratories, Toray Industries Inc.

• 今西 英世

  Basic Research Laboratories, Toray Industries Inc.

• 松本 修

  Basic Research Laboratories Toray Industries Inc.

• 今西 英世

  Basic Research Laboratories Toray Industries Inc.

関連論文

• Augmentation of Antibody-Dependent Cellular Cytotoxicity of Polymorphonuclear Leukocytes by Interferon-Gamma : Mechanism Dependent on Enhancement of Fc Receptor Expression and Increased Release of Activated Oxygens(Pharmacological)
• ENHANCEMENT OF IA-LIKE ANTIGEN EXPRESSION BY INTERFERON-GAMMA IN POLYMORPHONUCLEAR LEUKOCYTES(Communications to the Editor)
• Effects of Recombinant Human Interferon-Gamma (Met-Gln Form) on Expression of Fc Receptor and Ia-like Antigen on Human Peripheral Monocytes and Lymphocytes: A Comparative Study with Natural Human Interferon-Alpha and -Beta(Pharmacological)
• Effect of Interferon on Activated Oxygen Production in Human Monocytes in Vitro : Direct Measurement of Phagosomal Activated Oxygens by a New Method Using Luminol-Binding Microspheres
• Characteristics of Augmentation of Cell-Mediated Cytotoxic Activities by Recombinant Human Interferon-Gamma (Met-Glu Form) : A Comparative Study with Natural Human Interferon-Alpha and -Beta

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