Nuclear Magnetic Resonance Study on the Interaction of Aclacinomycin-A with a Deoxyribo-hexanucleotide Pentaphosphate d(CCTAGG)_2 in Aqueous Solution

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The interaction of aclacinomycin-A (ACM) with a deoxyribo-hexanucleotide pentaphosphate d(CCTAGG)_2 in aqueous solution was studied by ^1H, ^<31>P nuclear magnetic resonance techniques and by visible absorption spectroscopy. From the visible absorption spectra, ACM was found to be bound by polynucleotides with binding constants of the order of 10^6,in the order poly(dA-dT)>poly(dC)+poly(dG)>poly(dA-dG)+poly(dT-dC). With gradual addition of ACM to d(CCTAGG), two ^<31>P resonances newly appeared in the lower fied region as compared with d(CCTAGG) in the non-complex state, while the ^<31>P resonance assignable to the TpA portion in the non-complexed state decreased in intensity. From these results, the chromophore of ACM is expected to be intercalated in the TpA portion of the oligo-deoxyribonucleic acid duplex. The line widths of the exchangeable proton resonances of complex in H_2O were examined as a funciton of the temperature. The results suggested that ACM stabilizes the helix of d(CCTAGG)_2 at a region around the intercalation site.
社団法人日本薬学会の論文
1986-11-25