Effect of Protein-Bound Polysaccharide (PS-K) on Microtubule Proteins. III. Some Properties of PS-K Inhibition of Microtubule Polymerization and the Site of Its Action

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A protein-bound polysaccharide (PS-K) suppressed glycerol-free microtubule polymerization in the same way as it suppressed the polymerization in a reassembly mixture containing glycerol. However, the extent of inhibition was enhanced in the absence of glycerol. Maximal inhibition reached about 85% (the extent of polymerization was 15% of the original level) in the standard assembly medium containing 100mM KCl. The addition of PS-K increased the critical concentration for microtubule polymerization. Electron microscopy indicated that PS-K did not change the ultrastructure of microtubules. During incubation with PS-K, microtubule proteins retained their ability to polymerize, because the addition of taxol largely restored the inhibition by PS-K and the presence of an excess amount of microtubule-associated proteins (MAPs) or tubulin dimers also sequestered the inhibition to some extent. The extent of cancellation was greater by MAPs than by tubulin dimers.
公益社団法人日本薬学会の論文
1984-11-25