Analysis of 1,4-Dimorpholino-7-phenylpyrido [3,4-d] pyridazine (DS-511) and Its Metabolites in Biological Specimens. II. Fluorodensitometric Method for Simultaneous Determination of DS-511 and Its Metabolites in Plasma

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A simple and rapid fluorodensitometric method for the simultaneous determination of 1,4-dimorpholino-7-phenylpyrido [3,4-d] pyridazine (DS-511) and its metabolites in plasma was established. DS-511 and the metabolites were extracted from plasma with ethyl acetate. The organic layer was subjected to thin-layer chromatography with ethyl acetate-benzene (3 : 2). The air-dried chromatogram was moistened with 1-butanol, then the fluorescent spots were quantitatively determined with a spectro-densitometer in a fluorescence mode. A linear calibration plot for DS-511 was obtained in the concentration range of 0.02-0.50 μg per 1 ml of plasma. The accuracies as the coefficients of variation were 8 and 3% at concentrations of 0.05 and 0.25 μg of DS-511 per 1 ml of plasma, respectively. This method can be used to analyze plasma levels of DS-511 after its administration to rats and dogs, and should also be applicable to human plasma.
公益社団法人日本薬学会の論文
1979-10-25