Studies on Glutamic Oxalacetic Transaminase in the Rat. I. New Assay Procedure for Enzyme Activity in Crude Preparations

スポンサーリンク

概要

• 論文の詳細を見る

• Although the effects of various agents on glutamic oxalacetic transaminase (GOT) in tissue have been studied, the presence of isoenzymes, which have different characters, showed the lack for quantitativity in many of assay methods and the possibility of misleading results. In this report, the assay procedure for this enzyme activity in liver homogenate of rats was modified respect to the existence of isoenzymes. One procedure is a modified manometric method consisting of a coupled-system with chemical decarboxylation of oxalacetate to eliminate the inhibitory action of mitochondrial enzyme (m-GOT), and the other is a new type of spectrophotometric procedure. The latter could also be employed for the approximate calculation of isoenzyme levels in homogenates in combination with electrophoretic procedures for isoenzymes ratio with cellulose acetate film.

• 公益社団法人日本薬学会の論文
• 1968-10-25

著者

• 米谷 行男

  Shionogi Research Laboratories, Shionogi & Co., Ltd.,

• 馬場 良子

  Shionogi Research Laboratory Shionogi & Co. Ltd.

• 米谷 行男

  Shionogi Research Laboratories Shionogi & Co. Ltd.

• 小川 保直

  Shionogi Research Laboratory Shionogi And Co. Ltd.

関連論文

• Synthesis of 2,3-Dihydro-1,4-benzodioxin Derivatives. II. : 5(or 6)-Acyl 2,3-Dihydro-1,4-benzodioxin Derivatives : New Phenoxyacetic Acid Diuretics
• Synthesis of 2,3-Dihydro-1,4-benzodioxin Derivatives. I. : 2-Substituted-5(and 6)-sulfamoyl-2,3-dihydro-1,4-benzodioxins
• Origin of Plasma Uric Acid induced by l-Epinephrine
• Production of Uric Acid and Existence of Xanthine Oxidase in Rat Plasma
• Epinephrine-induced Hyperuricemia in Experimental Animals
• Studies on Glutamic Oxalacetic Transaminase in the Rat. III. Hormonal Control of Isoenzyme Levels in Liver by the Pituitary-Adrenal System
• Studies on Glutamic Oxalacetic Transaminase in the Rat. II. Selective Denaturation of Isoenzymes by Cellulose Acetate Treatment
• Studies on Glutamic Oxalacetic Transaminase in the Rat. I. New Assay Procedure for Enzyme Activity in Crude Preparations

スポンサーリンク