Pairing of Monomeric Units of Nucleic Acids. 1. Stoichiometric Interactions of Complementary Nucleosides in Methanol. Detection through Conductance Measurements (Studies on Heterocyclic Compounds.V.)

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With the object of obtaining informations about stoichiometry and specificity of interactions of unit monomers of nucleic acids in solution, specific conductance of mixed methanol solutions of nucleosides of various molar ratios ws measured. Maximum interactions were indicated by the minimum or the inflection point in the conductance-molar ratio relationships, and equimolar interactions were detected in the two component systems of uridine-adenosine, cytidine-adenosine, cytidine-guanosine, deoxythymidine-deoxyadenosine, and deoxycytidine-deoxyguanosine, and also in the three component systems of uridine-cytidine-gyanosine. No interaction was observed between uridine and guanosine, deoxythymidine and guanosine, or uridine and inosine. The whole experimental results were explainable consistently in terms of co-operative hydrogen bonding. Four types of the sets of two hydrogen bonds were classified, and it was clarified that while the hydrogen bond sets were consisted exclusively of N-H…O bond in the negative cases of interaction, N-H…N bond was common to the hydrogen bond sets of the systems which showed positive results. The cause of negative results was ascribed to the O-H…O bond formed between keto group of a base and surrounding methanol molecules, which might interfere with pairing of base moieties of nucleosides.
公益社団法人日本薬学会の論文
1967-04-25