The Role of Fe^<3+> on Fe^<2+> -Dependent Lipid Peroxidation in Phospholipid Liposomes

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概要

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• Fe^<2+>-dependent lipid peroxidation in phosphatidylcholine(PC)liposomes, assessed by thiobarbituric acidreactive substances(TBARS)production, was stimulated in the presence of Fe^<3+> in a concentration-dependent manner. The rates of nitroblue tetrazolium(NBT)reduction and Fe^<2+> oxidation(Fe^<2+> disappearance and Fe^<3+> formation)were also enhanced by the addition of Fe^<3+> to the reaction mixture, and there is a good linear relationship between these parameters. These results suggest that the facilitation of reactive oxygen species(ROS)production via Fe^<2+> oxidation is closely related to the onset of the stimulatory effect of Fe^<3+> on Fe^<2+>-dependent lipid peroxidation. On the other hand, results using the liposomes containing various concentrations of endogenous lipid hydroperoxides(LOOH)indicated that endogenous LOOH is not directly involved in the onset of the Fe^<3+> stimulatory effect on Fe^<2+>-depenent TBARS production and ROS production. This hypothesis was futher confirmed by the evidence that Fe^<2+>-dependent ROS production and Fe^<2+> oxidation of dipalmitoylphosphatidyl-choline liposomes were also stimulated by the addition of Fe^<3+>. The results with several antioxidants and radical scavengers suggested that ROS related to Fe^<2+>-dependent lipid peroxidation and its stimulation by Fe^<3+> are ferrous-oxygen complexes rather than superoxide anion, hydrogen peroxide and hydroxyl radicals. Based on these results, we proposed a possible mechanism for the onset of the Fe^<3+> stimulation in Fe^<2+>-dependent lipid peroxidation.

• 公益社団法人日本薬学会の論文
• 2002-02-01

著者

• Ohyashiki Takao

  Department Of Clinical Chemistry Faculty Of Pharmaceutical Sciences Hokuriku University

• Ohyashiki Takao

  Department Of Biochemistry Faculty Of Pharmaceutical Science Hokuriku University

• Kadoya Akinori

  Department Of Clinical Chemistry Faculty Of Pharmaceutical Sciences Hokuriku University

• KUSHIDA Katsumi

  Department of Clinical Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University

• Kushida K

  Department Of Clinical Chemistry Faculty Of Pharmaceutical Sciences Hokuriku University

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