Binding Properties of Adenosine Deaminase Interacted with Theophylline

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概要

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• Thermodynamic studies were carried out to evaluate the binding of theophylline on adenosine deaminase (ADA) in 50 mM sodium phosphate buffer pH 7.5, at 300 K, using isothermal titration calorimetry (ITC). A simple method for determination of binding isotherm in the drug-ADA interaction was applied using ITC data. ADA has two binding sites for theophylline, which show positive cooperativity in its sites. The intrinsic association equilibrium constants are 6 and 52 mм^<-1> in the first and second binding sites, respectively. Hence, occupation of the first site has produced an appreciable enhancement by 8.7 of the binding affinity of the second site. The molar enthalpies of binding are -12.2 and -14.9kJ/mol in the first and second binding sites, respectively.

• 公益社団法人日本薬学会の論文
• 2004-10-01

著者

• Saboury A

  Inst. Of Biochemistry And Biophysics Univ. Of Tehran

• Moosavi-movahedi Ali

  Institute Of Biochemistry And Biophysics

• Moosavi‐movahedi A

  Institute Of Biochemistry And Biophysics University Of Tehran

• Amanlou Masoud

  Institute Of Biochemistry And Biophysics University Of Tehran

• HAKIMELAHI Gholam

  TaiGen Biotechnology

• SABOURY Ali

  Institute of Biochemistry and Biophysics, University of Tehran

• BAGHERI Soghra

  Institute of Biochemistry and Biophysics, University of Tehran

• ATAIE Ghasem

  Faculty of Paramedical Science, Shahid-Beheshti University of Medical Science

• AMANLOU Masoud

  Department of Medical Chemistry, Tehran University of Medical Science

• HAKIMELAHI Gholam

  Institute of Chemistry, Academia Sinica Taipei

• CRISTALLI Gloria

  Department of Chemical Sciences, University of Camerino

• NAMAKI Saeid

  Faculty of Paramedical Science, Shahid-Beheshti University of Medical Science

• Saboury Ali

  Institute Of Biochemistry And Biophysics University Of Tehran

• Ataie Ghasem

  Faculty Of Paramedical Science Shahid-beheshti University Of Medical Science

• Namaki Saeid

  Faculty Of Paramedical Science Shahid-beheshti University Of Medical Science

• Bagheri Soghra

  Institute Of Biochemistry And Biophysics University Of Tehran

• Moosavi-movahedi Ali

  Institute Of Biochemistry & Biophysics University Of Tehran

• Cristalli Gloria

  Department Of Chemical Sciences University Of Camerino

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