Thermal Dissection of Lentil Seedling Amine Oxidase Domains by Differential Scanning Calorimetry

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概要

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• The relationships between the structural and energetic domains of lentil seedling amine oxidase (LSAO) were investigated using modifiers that target the active site and the carbohydrate moiety of the enzyme. An irreversible inhibitor, aminoguanidine, specifically modified the active site of the lentil enzyme, whereas sodium metaperiodate cleaves carbohydrate moieties covalently bound to the native enzyme. Differential scanning calorimetry (DSC) measurements were made on the modified LSAOs. Deconvolution of the reversible thermal DSC profiles of the modified enzyme gave three subpeaks (energetic domains), each of which was assigned to one of the three structural domains of the native protein. Our results led us to conclude that deglycosylation of LSAO has no effect on thermal stability, whereas binding of the inhibitor imparts more stability to the enzyme.

• 社団法人 日本農芸化学会の論文
• 2007-07-23

著者

• Saboury A

  Inst. Of Biochemistry And Biophysics Univ. Of Tehran

• Moosavi-movahedi Ali

  Institute Of Biochemistry And Biophysics

• Rezaei-tavirani Mostafa

  Institute Of Biochemistry And Biophysics University Of Tehran

• Moosavi‐movahedi A

  Institute Of Biochemistry And Biophysics University Of Tehran

• Amanlou Masoud

  Institute Of Biochemistry And Biophysics University Of Tehran

• Yousefi Reza

  Institute Of Biochemistry And Biophysics University Of Tehran

• Ahmad Faizan

  Centre For Interdiscipinary Research In Basic Sciences

• AMANI Mojtaba

  Institute of Biochemistry and Biophysics, University of Tehran

• FLORIS Giovanni

  Department of Applied Science in Biosystems, University of Cagliari

• MURA Anna

  Department of Applied Science in Biosystems, University of Cagliari

• MOOSAVI-NEJAD Seyedeh

  Institute of Biochemistry and Biophysics, University of Tehran

• HASHEMNIA Sedigheh

  Institute of Biochemistry and Biophysics, University of Tehran

• HAKIMELAHI Gholam

  TaiGen Biotechnology

• SABOURY Ali

  Institute of Biochemistry and Biophysics, University of Tehran

• Mura Anna

  Department Of Applied Science In Biosystems University Of Cagliari

• Saboury Ali

  Institute Of Biochemistry And Biophysics University Of Tehran

• Moosavi-movahedi Ali

  Institute Of Biochemistry & Biophysics University Of Tehran

• Floris Giovanni

  Department Of Applied Science In Biosystems University Of Cagliari

• Hashemnia Sedigheh

  Institute Of Biochemistry And Biophysics University Of Tehran

• Moosavi-nejad Seyedeh

  Institute Of Biochemistry And Biophysics University Of Tehran

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