Determination of Aromatic and Branched-Chain Amino Acids in Plasma by HPLC with Electrogenerated Ru (bpy)^<3+>_3 Chemiluminescence Detection

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概要

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• An HPLC method is described for the electrochemiluminescence (ECL) detection of amino acids, following cycloaddition reaction of their amino groups with divinyl sulfone (DVS), using electrogenerated tris (bipyridine) ruthenium (III). The derivatization reaction conditions were examined, with the optimum conditions found to be 40 mM DVS (pH 8.0) at 50℃ for 15min. Detection limits for the 15 amino acids examined varied greatly (0.04-8.0pmol) using a standard solution by flow injection analysis (FIA). These optimized conditions were used for HPLG determination of the amino acids in human plasma. A linear relationship was obtained up to 100pmol on a column for aromatic and branched-chain amino acids. Recoveries of Tyr, Met, Val, Leu, Ile, Phe and Trp when added to human plasma (1μmol/10ml plasma, n=5) were 101.5±1.1, 99.0±1.2, 98.0±1.4, 101.1±1.6, 95.1±1.6, 99.2±1.5 and 97.7±1.3 % (mean±S.D.) respectively. The concentrations of the amino acids in the plasma are in good agreement with other published data.

• 公益社団法人日本薬学会の論文
• 2003-09-01

著者

• Uchikura Kazuo

  College of Pharmacy, Nihon University

• Uchikura K

  Nihon Univ. Chiba

• Uchikura Kazuo

  College Of Pharmacy Nihon University

関連論文

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• Tris (2, 2'-bipyridine)ruthenium(III)Chemiluminescence Detection of Carbonyl Compounds with Methylmalonic Acid
• Ru(bpy)_3^ Electrochemiluminescence Detection of Aliphatic and Aromatic Amines with Diketene
• Electochemiluminescence Reaction by Mixing Tris(2, 2'-bipyridine)-ruthenium(III)with Ketones
• Determination of Aromatic and Branched-Chain Amino Acids in Plasma by HPLC with Electrogenerated Ru (bpy)^_3 Chemiluminescence Detection
• Direct enantiomeric separation of β-aminoalcohols with a tertiary amine moiety by ligand-exchange chromatography with chemiluminescence detection

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