Induction of Apoptosis by Saussurea lappa and Pharbitis nil on AGS Gastric Cancer Cells(Pharmacognosy)

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概要

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• We performed this study to understand the molecular basis underlying the antitumor effects of Saussurea lappa, Pharbitis nil, Plantago asiatica and Taraxacum mongolicum, which have been used for herbal medicinal treatments against cancers in East Asia. We analyzed the effects of these medicinal herbs on proliferation and on expression of cell growth/apoptosis related molecules, with using an AGS gastric cancer cell line. The treatments of Saussurea lappa and Pharbitis nil dramatically reduced cell viabilities in a dose and time-dependent manner, but Plantago asiatica and Taraxacum mongolicum didn't. FACS analysis and Annexin V staining assay also showed that both Saussurea lappa and Pharbitis nil induce apoptotic cell death of AGS. Expression analyses via RT-PCR and Western blots revealed that Saussurea lappa, but not Pharbitis nil, increased expression of the p53 and its downstream effector p21^<Waf1>, and that the both increased expression of apoptosis related Bax and cleavage of active caspase-3 protein. We also confirmed the translocation of Bax to mitochondria. Collectively, our data demonstrate that Saussurea lappa and Pharbitis nil induce growth inhibition and apoptosis of human gastric cancer cells, and these effects are correlated with down- and up-regulation of growth-regulating apoptotic and tumor suppressor genes, respectively.

• 公益社団法人日本薬学会の論文
• 2004-10-01

著者

• SHIN Min-Kyu

  Department of Physiology, College of Oriental Medicine, Kyunghee University

• Ko Seong-gyu

  Department Of Tumor Biology Cancer Research Institute College Of Medicine Seoul National University:

• BAE Hyun-Su

  Department of Physiology, College of Oriental Medicine, KyungHee University

• Bae Hyun-su

  Department Of Physiology College Of Oriental Medicine Kyunghee University

• JUN Chan-Yong

  Department of Internal Medicine, College of Oriental Medicine, Kyungwon University

• Nam Chang-gyu

  Department Of Internal Medicine College Of Oriental Medicine Semyung University

• Shin M‐k

  Department Of Physiology College Of Oriental Medicine Kyunghee University

• KOH Seung-Hee

  Department of Internal Medicine, College of Oriental Medicine, Kyungwon University

• Jun Chan-yong

  Dep. Of Internal Medicine Coll. Of Oriental Medicine Kyungwon Univ.

• Jun Chan-yong

  Department Of Internal Medicine College Of Oriental Medicine Kyungwon University

• Ko Seong-gyu

  Department Of Preventive Medicine College Of Korean Medicine Kyunghee University

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• Induction of Apoptosis by Saussurea lappa and Pharbitis nil on AGS Gastric Cancer Cells(Pharmacognosy)
• Inhibitory Effects of Paeonia suffruticosa on Allergic Reactions by Inhibiting the NF-kappaB/IkappaB-alpha Signaling Pathway and Phosphorylation of ERK in an Animal Model and Human Mast Cells

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