Estrogen Added Intermittently, but Not Continuously, Stimulates Differentiation and Bone Formation in SaOS-2 Cells

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概要

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• Although it is well established that estrogen inhibits bone resorption, its effects on bone formation remain controversial. We studied the effects of intermittent and continuous treatment with estrogen on bone formation in vitro using long term cultures of SaOS-2 cells under conditions that permit mineralization. SaOS-2 cells cultured in dexamethasone, ascorbic acid and β-glycerophosphate for up to 17 d formed mineralized bone nodules as visualized by von Kossa staining. Electron microscopic analysis of ultrathin sections of representative mineralized nodules showed the presence of mineral deposits, collagen fibrils and osteocytes. Both the mineralized nodule numbers and areas increased exponentially with time of culture after addition of β-glycerophosphate at day 8. Intermittent addition of 17 β-estradiol (E_2) for 6 h or 24 h of every 48 h starting at day 3 or day 8 to the end of culture period resulted in a specific time- and dose-dependent stimulation of mineralized bone nodule number and area, and alkaline phosphatase activity which were accompanied with increase in cell numbers. On the other hand, continuous treatment with E_2 added every 48 h had no effect. The estrogen receptor alpha (ERa) mRNA expression was stimulated after 6 or 24-h (intermittent), but not after 48-h (continuous) treatment with E_2. The stimulatory effect of E_2, when added intermittently, but not continuously, on differentiation and bone formation in human osteoblasts in culture may be relevant to previous reports of stimulatory effects of E2 on bone formation in vivo.

• 社団法人日本薬学会の論文
• 2003-07-01

著者

• Liu L

  Calcium Research Laboratory Division Of Endocrinology And Metabolism St. Michael's Hospital And

• RAO Leticia

  Calcium Research Laboratory, Division of Endocrinology and Metabolism, St. Michael's Hospital and De

• LIU Lily

  Calcium Research Laboratory, Division of Endocrinology and Metabolism, St. Michael's Hospital and De

• MURRAY Timothy

  Calcium Research Laboratory, Division of Endocrinology and Metabolism, St. Michael's Hospital and De

• McDERMOTT Elizabeth

  Department of Pathology, St. Michael's Hospital and Department of Medicine, University of Toronto

• ZHANG Xizhong

  Calcium Research Laboratory, Division of Endocrinology and Metabolism, St. Michael's Hospital and De

• Murray T

  Calcium Research Laboratory Division Of Endocrinology And Metabolism St. Michael's Hospital And

• Liu Lily

  Calcium Research Laboratory St.michael's Hospital Department Of Medicine University Of Toronto

• Rao Leticia

  Calcium Research Laboratory Division Of Endocrinology And Metabolism St. Michael's Hospital And

• Zhang X

  Calcium Research Laboratory Division Of Endocrinology And Metabolism St. Michael's Hospital And

• Murray Timothy

  Calcium Research Laboratory St.michael's Hospital Department Of Medicine University Of Toronto

• Zhang Xizhong

  Calcium Research Laboratory Division Of Endocrinology And Metabolism St. Michael's Hospital And

• Mcdermott Elizabeth

  Department Of Pathology St. Michael's Hospital And Department Of Medicine University Of Toronto

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